Differential Laser-Induced Perturbation Spectroscopy for Analysis of Mixtures of the Fluorophores l-Phenylalanine, l-Tyrosine and l-Tryptophan Using a Fluorescence Probe
| Autor: | Erman K. Oztekin, David W. Hahn |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Phenylalanine Analytical chemistry Mass spectrometry 01 natural sciences Biochemistry Fluorescence spectroscopy 010309 optics 03 medical and health sciences chemistry.chemical_compound 0103 physical sciences Partial least squares regression Aromatic amino acids Physical and Theoretical Chemistry Spectroscopy Fluorescent Dyes Chemistry Lasers Tryptophan General Medicine Fluorescence 030104 developmental biology Spectrometry Fluorescence Biophysics Tyrosine Fluorescence cross-correlation spectroscopy Biosensor |
| Zdroj: | Photochemistry and photobiology. 92(5) |
| ISSN: | 1751-1097 |
| Popis: | Quantitative detection of common endogenous fluorophores is accomplished using differential laser-induced perturbation spectroscopy (DLIPS) with a 193-nm UV fluorescence probe and various UV perturbation wavelengths. In this study, DLIPS is explored as an alternative to traditional fluorescence spectroscopy alone, with a goal of exploring natural fluorophores pursuant to biological samples and tissue analysis. To this end, aromatic amino acids, namely, l-phenylalanine, l-tyrosine and l-tryptophan are mixed with differing mass ratios and then classified with various DLIPS schemes. Classification with a traditional fluorescence probe is used as a benchmark. The results show a 20% improvement in classification performance of the DLIPS method over the traditional fluorescence method using partial least squares (PLS) analysis. Additional multivariate analyses are explored, and the relevant photochemistry is elucidated in the context of perturbation wavelengths. We conclude that DLIPS is a promising biosensing approach with potential for in vivo analysis given the current findings with fluorophores relevant to biological tissues. |
| Databáze: | OpenAIRE |
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