Quantitative reverse transcription-polymerase chain reaction of GABAA α1, β1 and γ2S subunits in epileptic rats following photothrombotic infarction of neocortex
| Autor: | Dennis R. Grayson, Elena A. Kharlamov, Kevin M. Kelly, Kathy L Schmitt, Carol Stolarski, Jiangping Liu |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Protein subunit Gene Expression Alpha (ethology) Neocortex Biology Cohort Studies Rats Sprague-Dawley Epilepsy Internal medicine Gene expression medicine Animals RNA Messenger Receptor Analysis of Variance Rose Bengal Reverse Transcriptase Polymerase Chain Reaction Cerebral Infarction Receptors GABA-A medicine.disease Rats Reverse transcription polymerase chain reaction medicine.anatomical_structure Endocrinology Real-time polymerase chain reaction Neurology Neurology (clinical) Intracranial Thrombosis |
| Zdroj: | Epilepsy Research. 52:85-95 |
| ISSN: | 0920-1211 |
| DOI: | 10.1016/s0920-1211(02)00194-8 |
| Popis: | Photothrombotic brain infarction can result in altered expression of cortical GABA(A) receptors and in epileptic seizures. We sought to determine whether infarct size and/or epileptic seizures resulted in a differential expression of cortical GABA(A) receptor subunit mRNA in adult rats. A reverse transcription-polymerase chain reaction (RT-PCR) was used with internal standards for GABA(A) receptor subunits to quantify alpha(1), beta(1), and gamma(2S) subunit mRNA expression in cortex ipsilateral and contralateral to left cerebral infarcts in small or large infarct/nonepileptic cohorts, a large infarct/epileptic cohort, and a young adult control cohort. Unilateral hemispheric subunit mRNA was pooled for each cohort, quantified, and expressed as mean values+/-S.E.M. In general, the magnitude of mRNA expression (pg/1 microg total RNA) was different for the individual subunits: gamma(2S) (10(4)), alpha(1) (10(2)), and beta(1) (10(1)). Hemispheric subunit mRNA expression for the different cohorts was compared by ANOVA testing, which noted significant differences for the alpha(1) (P |
| Databáze: | OpenAIRE |
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