An alternative to phosphotyrosine-containing motifs for binding to an SH2 domain
| Autor: | Yao-Zhong Lin, John P. Donahue, SongYi Yao, Mauricio Rojas |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Biophysics
Plasma protein binding Biology SH2 domain environment and public health Biochemistry src Homology Domains chemistry.chemical_compound Mice Animals Tyrosine Phosphorylation Phosphotyrosine Molecular Biology Adaptor Proteins Signal Transducing GRB2 Adaptor Protein Proteins Tyrosine phosphorylation Cell Biology 3T3 Cells chemistry biology.protein GRB2 biological phenomena cell phenomena and immunity Signal transduction hormones hormone substitutes and hormone antagonists Protein Binding Signal Transduction |
| Zdroj: | Biochemical and biophysical research communications. 234(3) |
| ISSN: | 0006-291X |
| Popis: | Shc is an important signalling protein whose overexpression leads to cell transformation in NIH 3T3 fibroblasts. Although the formation of Shc/Grb2 complexes involving Shc tyrosine residue 317 is necessary to induce this transformation, the Shc proteins in these Shc-overexpressing cells are not substantially tyrosine-phosphorylated. This observation led to our hypothesis that the non-phosphorylated Tyr317-containing region of Shc might have specific affinity for the Grb2 protein. We show here that cell-permeable peptides encompassing the Shc Tyr317 region, 312FDD-PSYVNVQNL323, can bind to the SH2 domain of Grb2 regardless of the state of tyrosine phosphorylation. When delivered into cells, both phosphorylated and non-phosphorylated Shc peptides inhibit growth factor-induced Shc/Grb2 protein-protein interaction. The non-phosphorylated Shc peptides with single point mutations at Asp313, Asp314, or Tyr317 are inactive, suggesting that these residues play an important role in Grb2 protein recognition. Our findings represent the first paradigm of the specific interaction between an unphosphorylated tyrosine-containing region and an SH2 domain and have important implications for understanding the mechanism of cell transformation by Shc overexpression. |
| Databáze: | OpenAIRE |
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