Characterization of the cellular antigens of Paracoccidioides brasiliensis yeast form
| Autor: | M Casotto |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Microbiology (medical)
Paracoccidioides brasiliensis Antigens Fungal biology medicine.diagnostic_test Paracoccidioidomycosis Paracoccidioides biology.organism_classification medicine.disease Histoplasmosis Microbiology Kinetics Mycoses Antigen Western blot Immunoglobulin G biology.protein medicine Humans Isoelectric Point Mitosporic Fungi Antibody Blastomycosis Research Article |
| Zdroj: | Journal of Clinical Microbiology. 28:1188-1193 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.28.6.1188-1193.1990 |
| Popis: | Antigenic components of the yeast extract of Paracoccidioides brasiliensis Linder 2511 cultured for 3, 8, 20, 30, and 60 days were examined by the Western blot (immunoblot) technique. The 3-day extract was chosen for characterization of the antigenic components because its stability did not vary with time and it contained all antigens identified by patient sera. Antibodies to cross-reacting antigens of P. brasiliensis extracts were detected in sera from patients with histoplasmosis, candidiasis, and aspergillosis. The 58-, 57-, 21-, and 16-kilodalton (kDa) antigens were specific for P. brasiliensis, while the 48- and 45-kDa antigens were specific for paracoccidioidomycosis. The Western blot technique is a useful tool for the diagnosis of disease and revealed heterogeneity in the responses of patient sera. The combination of the 58-, 57-, and 45-kDa proteins confirmed a diagnosis of paracoccidioidomycosis (87% of the cases). |
| Databáze: | OpenAIRE |
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