A pilot study to investigate the histomorphometric changes of murine maxillary bone around the site of mini-screw insertion in regenerated bone induced by anabolic reagents
| Autor: | Shigeki Nagahiro, Kazuhiro Aoki, Yoshiro Matsumoto, Yasuhiro Shimizu, Takashi Ono, Preksa Keo, Masaomi Ikeda |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Anabolism Bone Screws Orthodontics Pilot Projects Bone morphogenetic protein 2 03 medical and health sciences Mice 0302 clinical medicine In vivo Bone Density Injection site Maxillary first molar Maxilla Animals Bone mineral Chemistry business.industry 030206 dentistry Mice Inbred C57BL Fluorescent labelling 030104 developmental biology Coronal plane Indicators and Reagents Nuclear medicine business |
| Zdroj: | European journal of orthodontics. 43(1) |
| ISSN: | 1460-2210 |
| Popis: | Summary Objective The objective of this study was to investigate the histomorphometric changes around the site of mini-screw insertion in the regenerated bone which was induced by an anabolic-injection method using both anabolic peptide and bone morphogenetic protein 2 (BMP-2). Methods Twenty-seven eight-week-old C57BL/6J male mice were used. Some mice received submucosal co-injections of anabolic peptide and BMP-2 just in front of the maxillary first molar. Screw insertion was then performed 4 weeks after injection. All mice underwent a weekly in vivo micro-focal X-ray computed tomography (µCT) analysis before being sacrificed at week 8. The bone formation activity was evaluated using fluorescent labelling in the undecalcified sections. The analyses, including screw insertion, were performed in the frontal plane, in front of the site of screw insertion. Results Reconstructed µCT images revealed that the co-injection of anabolic reagents could lead to a gradual increase in the bone mineral density (BMD) of the injection-induced thickened bone by week 8. Both radiological and histomorphometric analyses indicated that screw insertion did not have any deleterious effects on either the BMD or the bone formation activity of the induced bone. Furthermore, the injection of anabolic reagents also led to an increase in the BMD of the underlying maxillary bone at the injection site. Conclusion Our histomorphometric analyses suggest that performing such anabolic injection to thicken bone could stimulate bone formation in the basal bone as well as in the induced bone. Similar augmentation of bone formation could be obtained even after subsequent screw insertion at the site of the induced bone. |
| Databáze: | OpenAIRE |
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