Molecular and histopathological profiling of imiquimod induced dermatosis in Swiss Wistar rats: contribution to the rat model for novel anti-psoriasis treatments
| Autor: | Jasmin Ramić, Lejla Pojskic, A Smajlovic, Anja Haverić, Naida Lojo-Kadric, Maida Hadžić, Amer Alić, Nikolina Elez-Burnjakovic, Ahmed Smajlović, Indira Mujezinović, Sanin Haverić |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Chemokine Down-Regulation Imiquimod Pharmacology Administration Cutaneous Betamethasone Severity of Illness Index Proinflammatory cytokine Ointments 03 medical and health sciences 0302 clinical medicine Psoriasis Genetics Medicine Animals Rats Wistar Receptor Molecular Biology Skin biology medicine.diagnostic_test business.industry General Medicine medicine.disease Rats Up-Regulation Gene expression profiling Disease Models Animal 030104 developmental biology Treatment Outcome 030220 oncology & carcinogenesis Skin biopsy biology.protein Cytokines business Transcriptome medicine.drug Signal Transduction |
| Zdroj: | Molecular biology reports. 48(5) |
| ISSN: | 1573-4978 |
| Popis: | Imiquimod (IMQ) induced human-like psoriasis in mice has been shown to be effective in testing and development of novel treatments. The IMQ psoriasis model has become widely used animal model, however, it is not completely characterized in different rat strains. We aimed to evaluate IMQ and betamethasone treatment for induction and reversal of psoriatic lesions on macroscopic, histological, genetic as well as cytokines and chemokines activation levels. Wistar rats were treated topically with IMQ. Adopted Psoriasis Area Severity Index (PASI) was calculated at the baseline, after the IMQ-symptoms induction and after betamethasone-symptoms reversal. Systematic effects were studied on cytokines and chemokines levels in plasma. Skin biopsy was taken to assess histological symptoms and selected inflammatory cytokines and receptors genes expression levels. Reversal of skin lesions, after betamethasone treatment, was significant (p = 0.03). Histological differences between untreated and IMQ-treated skin were significant for some markers (p |
| Databáze: | OpenAIRE |
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