Positive selection of activated T cells of the T8 (CD8) sub-type by immunomagnetic separation
| Autor: | Torbjørn Leivestad, Erik Thorsby, John Ugelstad, Gustav Gaudernack |
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| Rok vydání: | 2008 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte Cytotoxicity Immunologic Cell Survival medicine.drug_class T-Lymphocytes Immunology Population Receptors Cell Surface Transferrin receptor Cell Separation Biology Lymphocyte Activation Immunomagnetic separation Monoclonal antibody Biochemistry Magnetics Receptors Transferrin Genetics medicine Humans Immunology and Allergy Cytotoxic T cell education education.field_of_study General Medicine Molecular biology Antigens Surface biology.protein Radiobinding assay Antibody CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Tissue Antigens. 28:46-52 |
| ISSN: | 1399-0039 0001-2815 |
| Popis: | By conjugating a monoclonal IgM antibody of CD8-specificity to magnetite-containing polymer particles, we have developed a rapid and simple one-step procedure for positive selection of T8 cells. The method ensured good yield and viability of pure T8 cells. The positively selected T8-fraction from MLC activated T cells retained their cytolytic capacity and gave a moderate proliferative response. Most of the proliferative response occurred in the negatively selected T4 population which showed only a marginal cytotoxic activity against PHA blasts as target cells. Transferrin receptor expression, as measured in a direct radiobinding assay, could also be demonstrated both among pure T4 and T8 cells upon MLC-activation. |
| Databáze: | OpenAIRE |
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