Polyamines in the anemia of end-stage renal disease
Autor: | Janet C. Rice, David S. Kushner, Steven Chen, Fred Husserl, Christopher Della Santina, Barbara S. Beckman, L.y.n. Nguyen, James W. Fisher |
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Rok vydání: | 1991 |
Předmět: |
medicine.medical_specialty
Anemia Spermidine In Vitro Techniques End stage renal disease Pathogenesis Colony-Forming Units Assay chemistry.chemical_compound Bone Marrow hemic and lymphatic diseases Internal medicine medicine Polyamines Putrescine Humans Erythropoiesis Erythropoietin business.industry Continuous ambulatory peritoneal dialysis medicine.disease Endocrinology chemistry Nephrology Kidney Failure Chronic Spermine business Polyamine medicine.drug |
Zdroj: | Kidney international. 39(4) |
ISSN: | 0085-2538 |
Popis: | The improvement in the anemia in patients with end-stage renal disease (ESRD) on continuous ambulatory peritoneal dialysis (CAPD) suggests that dialyzable substances present in the sera of uremic patients either inhibit erythropoiesis directly or inactivate erythropoietin (EPO). In the present study predialysis sera from patients with ESRD inhibited erythroid colony (CFU-E) (N = 10) formation to a significantly (P less than 0.01) greater degree than granulocyte-macrophage (CFU-GM) (N = 7) colony formation in mouse bone marrow (MBM) cultures. The polyamines spermine (SP) (18 to 560 nm/ml) and spermidine (SD) (4 to 648 nm/ml) exerted a more significant (P less than 0.05) inhibition of CFU-E (N greater than or equal to 5) than that of CFU-GM (N greater than or equal to 5) growth. Concentrations of 0.80, 1.0, and 1.5 nm/ml of putrescine (PU) were 92%, 85%, and 77% of erythroid colony (CFU-E) controls (N = 4) and 104%, 130%, and 127% of CFU-GM controls (N = 4). Putrescine (PU) at 1.5 nm/ml also produced a significant (P less than 0.05) inhibition of CFU-E, whereas CFU-GM were stimulated by PU. These data suggest that predialysis sera from uremic patients, as well as SP, SD, and PU, are selectively more inhibitory to CFU-E than CFU-GM growth. The immunoreactivity of EPO was not significantly changed when it was coincubated with SP, SD and PU and measured by radioimmunoassay. PU was found to inhibit noncompetitively the bioactivity of EPO in a CFU-E assay. These data support the hypothesis that polyamines may be important uremic toxins in the anemia of ESRD. |
Databáze: | OpenAIRE |
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