Non‐targeted profiling of lipids during kainate‐induced neuronal injury
| Autor: | Guanghou Shui, Xin He, Xue Li Guan, Markus R. Wenk, Wee Kiang Yeo, Wei-Yi Ong |
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| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Ceramide Cannabinoid receptor Kainate receptor Ceramides Hippocampus Biochemistry Neuroprotection chemistry.chemical_compound Lipidomics Excitatory Amino Acid Agonists Genetics Animals Rats Wistar Molecular Biology Neurons Sphingolipids Kainic Acid Chemistry Phosphatidylethanolamines Glutamate receptor Brain Lipid metabolism Anandamide Lipid Metabolism Lipids Rats lipids (amino acids peptides and proteins) Biotechnology |
| Zdroj: | The FASEB Journal. 20:1152-1161 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | Kainate is a glutamate analog that has been widely used in pharmacological studies of neuronal injury related to ischemic conditions and epilepsy. While altered lipid metabolism has been implicated in kainate action, no study has yet investigated the associated changes in lipid metabolites on a systems scale. Here we describe a mass spectrometry-based approach for profiling of lipid mixtures in a nontargeted fashion. Combined with tandem mass spectrometry, this method aims to identify lipids that are altered between two conditions, the kainate-treated and the control hippocampal tissues. In addition to reductions in major phospholipids with mainly polyunsaturated fatty acyl chains, we find elevated levels of ions that correspond to acylated forms of phosphatidylethanolamines and ceramides. Acylated phosphatidylethanolamines are neuroprotective lipids and precursors for anandamide, which signals via cannabinoid receptors. Quantitative analysis of ceramides shows that many molecular species with different acyl compositions are increased during kainate treatment. This increase is mainly restricted to neurons rather than other brain cells in the hippocampus as revealed by immunohistochemistry of brain slices. |
| Databáze: | OpenAIRE |
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