A new sensitive and fast peptide immunoassay based on enzyme amplification used in the determination of CGRP and the demonstration of its presence in the thyroid

Autor:	Sunil J. Wimalawansa, Colin H. Self, S. I. Girgis, Axel Johannsson, David L. Bates, Iain MacIntyre
Rok vydání:	1985
Předmět:	Physiology Calcitonin Gene-Related Peptide Radioimmunoassay Thyroid Gland Nerve Tissue Proteins Peptide Biology Calcitonin gene-related peptide Biochemistry Immunoenzyme Techniques Cellular and Molecular Neuroscience Endocrinology medicine Animals Chromatography High Pressure Liquid chemistry.chemical_classification Antiserum medicine.diagnostic_test Immune Sera Microchemistry Thyroid Rats medicine.drug_formulation_ingredient Enzyme medicine.anatomical_structure chemistry Immunoassay Thyroid extract
Zdroj:	Peptides. 6:627-630
ISSN:	0196-9781
Popis:	An enzyme amplified immunoassay for rCGRP based on cofactor cycling has been found to be clearly superior to a comparable radioimmunoassay employing the same antiserum in terms of sensitivity, speed and convenience. Correlation between the two methods was very good. With the enzyme amplified immunoassay we have been able to demonstrate the existence of rCGRP in thyroid extract.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c9879db38f47d3e90663e3de88cc37d https://doi.org/10.1016/0196-9781(85)90164-0 Zobrazit plný text záznamu