Trileaflet aortic valve reconstruction with a decellularized pericardial patch in a sheep model
| Autor: | David I. Rhodes, William M L Neethling, Bart Meuris, Stéphanie De Vleeschauwer, Peter Verbrugghe, Shigeyuki Ozaki, Geoff Strange, Eric Verbeken |
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| Rok vydání: | 2016 |
| Předmět: |
Pulmonary and Respiratory Medicine
Aortic valve medicine.medical_specialty Hemodynamics Regurgitation (circulation) 030204 cardiovascular system & hematology law.invention 03 medical and health sciences 0302 clinical medicine Aortic valve repair law Internal medicine Cardiopulmonary bypass Animals Medicine Sheep Domestic Heart Valve Prosthesis Implantation Decellularization business.industry medicine.disease Surgery Disease Models Animal medicine.anatomical_structure 030228 respiratory system Echocardiography Aortic Valve Heart Valve Prosthesis Cardiology Implant Cardiology and Cardiovascular Medicine business Pericardium Calcification |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 152:1167-1174 |
| ISSN: | 0022-5223 |
| DOI: | 10.1016/j.jtcvs.2016.05.024 |
| Popis: | Background The purpose of this study was to provide a preliminary assessment of the performance of a decellularized pericardial patch in a trileaflet aortic valve reconstruction in a long-term juvenile sheep model. Methods A sheep surgical model was used to perform a complete trileaflet reconstruction (Ozaki technique) of the aortic valve with 3 separate pericardial patches. Valve function was assessed 1 week, 3 months, and 6 months after surgery via transthoracic echocardiography. Calcification resistance and host cell infiltration of the pericardial material were assessed at 6 months after surgery. Results Three of 6 sheep with implanted pericardial neo-cusps survived until the planned time of sacrifice after surgery, whereas 3 animals had a successful implant but died shortly after the procedure as the result of a bad recovery from cardiopulmonary bypass. Echocardiography at 6 months revealed a high coaptation area with only minimal regurgitation. In all explanted leaflets, cusp tissue was soft. There was only minimal calcification in 8 of 9 leaflets. Conclusions Aortic valves reconstructed with a decellularized pericardial patch demonstrated adequate diastolic function with minimal regurgitation and resistance to calcification. Combining the Ozaki technique with this decellularized pericardial scaffold showed adequate hemodynamics, sustained mechanical integrity of the patch and limited calcification of the material. These results, together with earlier experimental and clinical data, indicate the potential of this material for aortic valve repair. |
| Databáze: | OpenAIRE |
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