Properties and genetic control of four methyltransferases involved in methylation of anthocyanins in flowers of Petunia hybrida

Autor:	AndréW. Schram, J. E. Poulton, Mirjam E. G. Aarsman, L. M. V. Jonsson
Rok vydání:	1984
Předmět:	Methyltransferase biology Chromatofocusing food and beverages Plant Science Methylation biology.organism_classification chemistry.chemical_compound Isoelectric point chemistry Biochemistry Anthocyanin Genetics Delphinidin Gene Solanaceae
Zdroj:	Planta. 160:174-179
ISSN:	1432-2048 0032-0935
DOI:	10.1007/bf00392867
Popis:	Four S-adenosyl-L-methionine:anthocyanin-3',5'-O-methyltransferases in flowers of Petunia hybrida were separated using the chromatofocusing technique. Each methyltransferase is controlled by one of the methylation genes Mt1, Mt2, Mf1 or Mf2. Molecular weight, pH-activity optimum, isoelectric point, several kinetic properties and the behaviour in the presence of Mg(2+), ethylenediaminetetraacetic acid and S-adenosyl-L-homocysteine of each of the four enzymes were determined. The methylation in vitro of delphinidin 3-(p-coumaroyl)-rutinosido-5-glucoside reflected the accumulation patterns of methylated anthocyanins in vivo and established the regulatory role of methyltransferases in vivo.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c975570c44e7a5945f7b11cf6c4fccd https://doi.org/10.1007/bf00392867 Zobrazit plný text záznamu Full text from SpringerLink