Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway
| Autor: | Xiu-lan Su, Ying-xu Shi, Liya Su, Yaguang Xi, Mei-rong Yan |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Colorectal cancer Colon Poly ADP ribose polymerase Mice Nude Antineoplastic Agents Apoptosis Pharmacology Medicine Animals Humans Pharmacology (medical) Colorectal tumor Cell Proliferation business.industry Cell growth Rectum General Medicine medicine.disease HCT116 Cells Cancer research Myeloid Cell Leukemia Sequence 1 Protein Original Article Signal transduction Poly(ADP-ribose) Polymerases Tumor Suppressor Protein p53 business Colorectal Neoplasms Peptides Signal Transduction |
| Zdroj: | Acta pharmacologica Sinica. 36(12) |
| ISSN: | 1745-7254 |
| Popis: | We have reported novel anticancer bioactive peptides (ACBPs) that show tumor-suppressive activities in human gastric cancer, leukemia, nasopharyngeal cancer, and gallbladder cancer. In this study, we investigated the effects of ACBPs on human colorectal cancer and the underlying mechanisms.Cell growth and apoptosis of human colorectal tumor cell line HCT116 were measured using cell proliferation assay and flow cytometry, respectively. The expression levels of PARP, p53 and Mcl1A were assessed with Western blotting and immunohistochemistry. For evaluation of the in vivo antitumor activity of ACBPs, HCT116 xenograft nude mice were treated with ACBPs (35 μg/mL, ip) for 10 days.Treatment of HCT116 cells with ACBPs (35 μg/mL) for 4-6 days significantly inhibited the cell growth. Furthermore, treatment of HCT116 cells with ACBPs (35 μg/mL) for 6-12 h significantly enhanced UV-induced apoptosis, increased the expression of PARP and p53, and decreased the expression of Mcl-1. Administration of ACBPs did not change the body weight of HCT116 xenograft nude mice, but decreased the tumor growth by approximately 43%, and increased the expression of PARP and p53, and decreased the expression of Mcl-1 in xenograft mouse tumor tissues.Administration of ACBPs inhibits human colorectal tumor cell growth and induces apoptosis in vitro and in vivo through modulating the PARP-p53-Mcl-1 signaling pathway. |
| Databáze: | OpenAIRE |
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