| Popis: |
A survey was conducted to determine the diversity and frequency of endemic entomopathogenic nematodes (EPN) in fruit orchards in Rajgarh, Hamachi Pradesh provinces of India. The main aim of the survey was to obtain nematodes as biological control agents against tomato leafminer (TLM) Tuta absoluta, an invasive pest of tomato crop in India. From a total of 43 samples, tested positive for the presence of EPN. Sequencing and characterisation of the internal transcribed spacer (ITS) region was used to identify all nematode isolates to species level. Laboratory bioassays, use nematodes S. feltiae (HR1) and H. bacteriophora (HR2), species dose. The (%) 1 st instar larval mortality rate was ranged from 24.15 to 85.38 %. 2 nd instar from 29.87 to 90.00 %. 3 rd instar from 24.15 to 90.00 %. 4 th instar from 31.53 to 90.00 % and pupa stage mortality was ranged from 31.53 to 85.38 %. Larvicidal activity after 48- and 72-hours exposure time, the HR1 and HR2 showed potent larvicidal activity with LC50, LC75 and LC90 of all instars and pupa show high mortality rate. |
