Photolysis of 2-azidofluorene in situ as a probe in chemical carcinogenesis: bypass of requirement for metabolic activation
| Autor: | Awni M. Sarrif, William E. White, Nick DiVito |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Drug
In situ Azides Cell Survival Ultraviolet Rays media_common.quotation_subject Biophysics medicine.disease_cause Biochemistry Cell Line medicine Cytotoxicity Molecular Biology Biotransformation media_common Fluorenes Photolysis Photoaffinity labeling Chemistry Photodissociation Affinity Labels Cell Biology DNA Transformation (genetics) Kinetics Toxicity Carcinogens Spectrophotometry Ultraviolet Carcinogenesis |
| Zdroj: | Biochemical and biophysical research communications. 83(2) |
| ISSN: | 0006-291X |
| Popis: | 2-Azidofluorene was synthesized to serve as a specific photoaffinity label in chemical carcinogenesis studies. The drug exhibited little toxicity, and was non-transforming in the C3H 10T 1 2 CL8 cells. When photolysed at 360 nm in water to generate N-hydroxy-2-aminofluorene, then added to the cells, the drug again exhibited little toxicity but was very weakly transforming. When it was irradiated inside the cells cytotoxicity was enhanced greatly. Both Types II and III transformed foci were observed in transformation experiments and were comparable to those obtained with N-acetoxy-2-acetylaminofluorene. Irradiation alone was neither toxic nor transforming. These results suggest that photoaffinity labeling is a promising tool in studying chemical carcinogenesis by bypassing metabolic activation. |
| Databáze: | OpenAIRE |
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