Circular RNA circFOXP1 promotes angiogenesis by regulating microRNA -127-5p/CDKN2AIP signaling pathway in osteosarcoma
Autor: | Ziliang Yu, Bingbing Wu, Farui Sun, Haiping Zhang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Adult
musculoskeletal diseases Angiogenesis Mice Nude Bioengineering Applied Microbiology and Biotechnology Metastasis angiogenesis cdkn2aip Circular RNA Cell Line Tumor osteosarcoma microRNA medicine Animals Humans neoplasms Aged Cell Proliferation Tube formation Mice Inbred BALB C Base Sequence Neovascularization Pathologic Chemistry RNA-Binding Proteins RNA Circular General Medicine Middle Aged medicine.disease Gene Expression Regulation Neoplastic Survival Rate MicroRNAs Cancer research Immunohistochemistry Osteosarcoma mir-127-5p Signal transduction Apoptosis Regulatory Proteins circfoxp1 TP248.13-248.65 Research Article Research Paper Signal Transduction Biotechnology |
Zdroj: | Bioengineered, Vol 12, Iss 2, Pp 9991-9999 (2021) Bioengineered article-version (VoR) Version of Record |
ISSN: | 2165-5987 2165-5979 |
Popis: | Osteosarcoma is known to have a high metastatic potential, which is closely related to angiogenesis. circRNAs are closely associated with osteosarcoma metastasis. This study aims to investigate the role of Circular RNA circFOXP1 in angiogenesis in osteosarcoma. We detected circFOXP1 expression in osteosarcoma, as well as its prognostic value. Tube formation assay and immunohistochemistry staining were conducted to determine the condition of tube formation. RT-qPCR was performed to explore targeted genes. Luciferase reporter assays were carried out to explore the interaction between miR-127-5p, ircFOXP1, and CDKN2AIP, respectively. In vivo studies further confirmed the relationship between circFOXP1 and tumor angiogenesis in osteosarcoma. We found that circFOXP1 expression was increased in osteosarcoma, and could promote angiogenesis in osteosarcoma through upregulating CDKN2AIP expression. Moreover, circFOXP1 could directly bind to miR-127-5p, which further targets CDKN2AIP directly. In conclusion, circFOXP1 promoted angiogenesis by regulating miR-127-5p/CDKN2AIP signaling pathway in osteosarcoma. |
Databáze: | OpenAIRE |
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