Yeast-secreted, dried and food-admixed monomeric IgA prevents gastrointestinal infection in a piglet model
| Autor: | Erik Vanderbeke, Nico Callewaert, Ann Depicker, Vikram Virdi, Stefan Ryckaert, Jorge Palaci, Eric Cox, Bram Laukens |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
ENZYME
Gastrointestinal Diseases Swine Biomedical Engineering Administration Oral Bioengineering medicine.disease_cause Communicable Diseases Applied Microbiology and Biotechnology Article Pichia pastoris Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Enterotoxigenic Escherichia coli Escherichia coli medicine Animals Humans 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology fungi food and beverages Biology and Life Sciences Single-Domain Antibodies biology.organism_classification Yeast Immunoglobulin A 3. Good health Enzyme Monomer PICHIA-PASTORIS chemistry Food biology.protein Molecular Medicine Antibody 030217 neurology & neurosurgery Biotechnology Camelid |
| Zdroj: | NATURE BIOTECHNOLOGY Nature biotechnology |
| ISSN: | 1087-0156 1546-1696 |
| Popis: | Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within food. A single-gene-encoded monomeric IgA protects piglets from enterotoxic E. coli infection. |
| Databáze: | OpenAIRE |
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