TheC. elegansmodel in toxicity testing
Autor: | Piper Reid Hunt |
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Rok vydání: | 2016 |
Předmět: |
Animal Use Alternatives
0301 basic medicine Review Article predictive toxicology Predictive toxicology Computational biology 010501 environmental sciences Toxicology 01 natural sciences Median lethal dose Lethal Dose 50 03 medical and health sciences Toxicity Tests Animals Caenorhabditis elegans Hazard evaluation Review Articles Safety testing 0105 earth and related environmental sciences biology screening good C. elegans culture practice biology.organism_classification alternative model 030104 developmental biology Toxicity WHOLE ANIMAL toxin ranking |
Zdroj: | Journal of Applied Toxicology |
ISSN: | 1099-1263 0260-437X |
Popis: | Caenorhabditis elegans is a small nematode that can be maintained at low cost and handled using standard in vitro techniques. Unlike toxicity testing using cell cultures, C. elegans toxicity assays provide data from a whole animal with intact and metabolically active digestive, reproductive, endocrine, sensory and neuromuscular systems. Toxicity ranking screens in C. elegans have repeatedly been shown to be as predictive of rat LD50 ranking as mouse LD50 ranking. Additionally, many instances of conservation of mode of toxic action have been noted between C. elegans and mammals. These consistent correlations make the case for inclusion of C. elegans assays in early safety testing and as one component in tiered or integrated toxicity testing strategies, but do not indicate that nematodes alone can replace data from mammals for hazard evaluation. As with cell cultures, good C. elegans culture practice (GCeCP) is essential for reliable results. This article reviews C. elegans use in various toxicity assays, the C. elegans model's strengths and limitations for use in predictive toxicology, and GCeCP. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. Journal of Applied Toxicology published by John Wiley & Sons Ltd. Toxicity ranking screens in Caenorhabditis elegans have repeatedly been shown to be as predictive of rat LD50 ranking as mouse LD50 ranking. Additionally, many instances of conservation of mode of toxic action have been noted between C. elegans and mammals. These consistent correlations make the case for inclusion of C. elegans assays in early safety testing and as one component in tiered or integrated toxicity testing strategies. Good C. elegans culture practice (GCeCP) is essential for reliable results. |
Databáze: | OpenAIRE |
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