Combination of Ruthenium Dendrimers and Acoustically Propelled Gold Nanowires as a Platform for Active Intracellular Drug Delivery Towards Breast Cancer Therapy
| Autor: | Sylwia Michlewska, Gulcin Bolat, Paula Ortega, Zuzana Garaiova, Francisco Javier de la Mata, Natalia Sanz del Olmo, Tibor Hianik, Hua Gong, Berta Esteban-Fernández de Ávila, Joseph Wang |
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| Rok vydání: | 2019 |
| Předmět: |
Chemistry
Graphene media_common.quotation_subject Nanowire chemistry.chemical_element 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Ruthenium law.invention law Dendrimer Biophysics General Earth and Planetary Sciences Nanomotor 0210 nano-technology Internalization Intracellular General Environmental Science media_common |
| Zdroj: | Clinical Oncology and Research |
| ISSN: | 2613-4942 |
| DOI: | 10.31487/j.cor.2019.04.08 |
| Popis: | In this work, a new class of fluorescently labeled metallodendrimers based on ruthenium and possessing anticancer activity (FITC-CRD13) is combined with graphene oxide modified gold nanowires (GO-AuNWs). The resulting complexes were tested as active intracellular transporters being propelled by ultrasound field (US) and using breast cancer cells as a model. Energy dispersive X-ray spectroscopy analysis confirmed the successful modification of GO-AuNWs by dendrimers as shown by the uniform presence of ruthenium over the nanomotor structure corresponding to the ruthenium groups of FITC-CRD13. The binding of dendrimers to the surface of GO-AuNWs was accompanied by quenching their fluorescence signal. Upon the application of an ultrasound field (5 min, 2 V, 2.66 MHz), the complexes were propelled towards MCF7 breast cancer cells, detaching from the GO-nanomotor surface and thus recovering the dendrimer fluorescence signal. Fluorescence signal from US-treated samples was ~1.8 fold higher compared to passive controls. The results obtained in this work suggest that US-propelled AuNWs lead to faster cell internalization, hence accelerating the delivery of the carbosilane ruthenium dendrimers (CRD) payload inside MCF7 cells. |
| Databáze: | OpenAIRE |
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