Preferential diaphragmatic weakness during sustained Pseudomonas aeruginosa lung infection
| Autor: | Stephanie A. Tuck, Weisheng Bao, Danuta Radzioch, Basil J. Petrof, Maziar Divangahi, Stefan Matecki, Alain S. Comtois, Roy W. R. Dudley |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male Pathology medicine.medical_specialty Time Factors Diaphragm Colony Count Microbial Inflammation Critical Care and Intensive Care Medicine Sepsis Mice medicine Respiratory muscle Pneumonia Bacterial Animals Pseudomonas Infections Diaphragmatic weakness Respiratory system Muscle Skeletal Lung Respiratory Tract Infections Muscle Weakness medicine.diagnostic_test business.industry Respiratory disease medicine.disease Hindlimb Mice Inbred C57BL Disease Models Animal Bronchoalveolar lavage medicine.anatomical_structure Muscle Fibers Slow-Twitch Immunology Muscle Fibers Fast-Twitch Respiratory Mechanics medicine.symptom business Muscle Contraction |
| Zdroj: | American journal of respiratory and critical care medicine. 169(6) |
| ISSN: | 1073-449X |
| Popis: | Infection with Pseudomonas aeruginosa plays a major role in the pulmonary inflammation and injury associated with cystic fibrosis. Lung inflammation may also lead to more widespread systemic effects on other organs. We tested the following hypotheses: (1) ongoing P. aeruginosa lung infection produces diaphragmatic and limb muscle weakness and (2) such muscle dysfunction is directly correlated with the level of pulmonary inflammation. Chronic bronchopulmonary infection with mucoid P. aeruginosa was induced in C57BL/6 mice. At Day 2 after infection, diaphragmatic force was decreased (37%) only in mice infected with a high dose of 1 x 10(6) cfu, whereas by Day 7 after infection, diaphragmatic force was similarly reduced (36%) even at a fivefold lower inoculating dose. No significant correlations were found between diaphragmatic weakness and pulmonary inflammation, as assessed by the number of neutrophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid. Moreover, in marked contrast to the diaphragm, no effects of P. aeruginosa infection on contractile function were observed in prototypical slow- and fast-twitch hindlimb muscles. We conclude that sustained lung infection with P. aeruginosa induces preferential weakness of the diaphragm, which is not directly correlated with the degree of pulmonary inflammation induced under these conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|