An explorative study of polymers for 3D printing of bioanalytical test systems
Autor: | Christoph Jurischka, Franziska Dinter, Juliane Schiebel, Anastasia Efimova, Thomas Fischer, Peter Schierack, Stefan Rödiger, Romano Weiss, Bekzodjon Fayziev, Christian Schulz |
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Přispěvatelé: | Publica |
Rok vydání: | 2020 |
Předmět: |
Bioanalysis
Materials science Polymers Physiology Nanotechnology autofluorescence 030204 cardiovascular system & hematology Fluorescence spectroscopy 030218 nuclear medicine & medical imaging 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Differential scanning calorimetry Polylactic acid Physiology (medical) Sample preparation thermoplastic polyurethane elastomers chemistry.chemical_classification 3D printing Hematology Polymer Autofluorescence chemistry Printing Three-Dimensional Drug delivery Cardiology and Cardiovascular Medicine |
Zdroj: | Clinical Hemorheology and Microcirculation. 75:57-84 |
ISSN: | 1875-8622 1386-0291 |
Popis: | Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement. |
Databáze: | OpenAIRE |
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