Lens-Specific Regulation of the Thioredoxin-1 Gene, but Not Thioredoxin-2, upon in Vivo Photochemical Oxidative Stress in the Emory Mouse
| Autor: | Pabbathi G. Reddy, Kailash C. Bhuyan, Durga K. Bhuyan |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Aging DNA Complementary animal structures Photochemistry Biophysics Biology medicine.disease_cause Biochemistry Cataract Mice Thioredoxins Western blot Downregulation and upregulation In vivo Complementary DNA Lens Crystalline medicine Animals Humans Tissue Distribution RNA Messenger Cloning Molecular Molecular Biology Gene medicine.diagnostic_test Cell Biology Molecular biology Disease Models Animal Oxidative Stress medicine.anatomical_structure Gene Expression Regulation Lens (anatomy) Female Thioredoxin Oxidation-Reduction Oxidative stress |
| Zdroj: | Biochemical and Biophysical Research Communications. 265:345-349 |
| ISSN: | 0006-291X |
| Popis: | Thioredoxin (TRX)-1 and TRX-2 redox-regulatory genes were analyzed in the lens and some other tissues of the Emory mouse, a model for age-related human cataract. The cDNA transcripts of mRNAs encoding TRX-1 and TRX-2 genes were isolated and cloned by RT-PCR from the lens, liver, kidney, and tail, and the cDNA sequences were similar to the reported sequences of murine TRX-1 and TRX-2 genes. In vivo photochemical oxidative stress to the Emory mice resulted in fivefold upregulation of the lens TRX-1 gene at 3 weeks and declined thereafter. Western blot analysis revealed a fourfold increase of TRX-1 protein in the lens at 3 weeks after oxidative stress. The TRX-2 gene in the lens was not changed up to 5 weeks and decreased by 50% thereafter. However, the expressions of these genes in the liver, kidney, and tail were not changed. Fluorescent light or riboflavin alone did not affect the expressions of TRX-1 and TRX-2 genes in the lens. Thus, we show the expressions of TRX-1 and TRX-2 genes in the lens, liver, kidney, and tail and lens-specific upregulation of the TRX-1 gene and protein expressions, possibly as a protective response to the altered redox state of the lens after in vivo oxidative stress to the Emory mouse. |
| Databáze: | OpenAIRE |
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