Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library
| Autor: | Lee Makowski, Toshiyuki Mori, Kathryn M. Schumacher, James B. McMahon, Lauren R. H. Krumpe |
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| Rok vydání: | 2007 |
| Předmět: |
Phage display
T7 phage lcsh:Biotechnology viruses Phagemid Molecular Sequence Data Peptide 03 medical and health sciences 0302 clinical medicine Trinucleotide Repeats Peptide Library lcsh:TP248.13-248.65 Bacteriophage T7 Amino Acid Sequence Peptide library Peptide sequence 030304 developmental biology Genetics chemistry.chemical_classification 0303 health sciences biology Methodology Article Genetic Variation biology.organism_classification Amino acid Mutagenesis Insertional Lytic cycle chemistry 030220 oncology & carcinogenesis Streptavidin Peptides Bacteriophage M13 Biotechnology |
| Zdroj: | BMC Biotechnology BMC Biotechnology, Vol 7, Iss 1, p 65 (2007) |
| ISSN: | 1472-6750 |
| DOI: | 10.1186/1472-6750-7-65 |
| Popis: | Background Amino acid sequence diversity is introduced into a phage-displayed peptide library by randomizing library oligonucleotide DNA. We recently evaluated the diversity of peptide libraries displayed on T7 lytic phage and M13 filamentous phage and showed that T7 phage can display a more diverse amino acid sequence repertoire due to differing processes of viral morphogenesis. Methods In this study, we evaluated and compared the diversity of a 12-mer T7 phage-displayed peptide library randomized using codon-corrected trinucleotide cassettes with a T7 and an M13 12-mer phage-displayed peptide library constructed using the degenerate codon randomization method. Results We herein demonstrate that the combination of trinucleotide cassette amino acid codon randomization and T7 phage display construction methods resulted in a significant enhancement to the functional diversity of a 12-mer peptide library. This novel library exhibited superior amino acid uniformity and order-of-magnitude increases in amino acid sequence diversity as compared to degenerate codon randomized peptide libraries. Comparative analyses of the biophysical characteristics of the 12-mer peptide libraries revealed the trinucleotide cassette-randomized library to be a unique resource. Conclusion The combination of T7 phage display and trinucleotide cassette randomization resulted in a novel resource for the potential isolation of binding peptides for new and previously studied molecular targets. |
| Databáze: | OpenAIRE |
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