Actin Dynamics Drive Microvillar Motility and Clustering during Brush Border Assembly
| Autor: | Bryan A. Millis, Caroline S. Cencer, Matthew J. Tyska, Meagan M. Postema, Leslie M. Meenderink, Evan S. Krystofiak, Isabella M. Gaeta, Colbie R. Chinowsky |
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| Rok vydání: | 2019 |
| Předmět: |
Brush border
Swine Motility Myosins Biology General Biochemistry Genetics and Molecular Biology EPS8 03 medical and health sciences 0302 clinical medicine Cell Movement Live cell imaging medicine Animals Humans Molecular Biology Actin Adaptor Proteins Signal Transducing 030304 developmental biology 0303 health sciences Microvilli Microfilament Proteins Cell Differentiation Epithelial Cells Cell Biology Cadherins Actins Epithelium Cell biology Brush border assembly HEK293 Cells Treadmilling medicine.anatomical_structure Caco-2 Cells 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Cell. 50:545-556.e4 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2019.07.008 |
| Popis: | Summary Transporting epithelial cells generate arrays of microvilli, known as a brush border, to enhance functional capacity. To understand brush border formation, we used live cell imaging to visualize apical remodeling early in this process. Strikingly, we found that individual microvilli exhibit persistent active motility, translocating across the cell surface at ∼0.2 μm/min. Perturbation with inhibitors and photokinetic experiments revealed that microvillar motility is driven by actin assembly at the barbed ends of core bundles, which in turn is linked to robust treadmilling of these structures. Actin regulatory factors IRTKS and EPS8 localize to the barbed ends of motile microvilli, where they control the kinetics and nature of movement. As the apical surface of differentiating epithelial cells is crowded with nascent microvilli, persistent motility promotes collisions between protrusions and ultimately clustering and consolidation into higher-order arrays. Thus, microvillar motility represents a previously unrecognized driving force for apical surface remodeling and maturation during epithelial differentiation. |
| Databáze: | OpenAIRE |
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