Growth inhibitory effect of 4-phenyl butyric acid on human gastric cancer cells is associated with cell cycle arrest
| Autor: | Meng-Wan Song, Jing Tao, Long-Zhu Li, Bingxiu Xiao, Xue-Yan Sun, Hongxia Deng, Wen-Zhu Lou, Junming Guo |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Cell cycle checkpoint Brief Article Cellular differentiation Antineoplastic Agents Phenylbutyrate Flow cytometry Butyric acid chemistry.chemical_compound Stomach Neoplasms Internal medicine Cell Line Tumor parasitic diseases medicine Humans Cell Proliferation medicine.diagnostic_test Cell growth Chemistry Cell Cycle Gastroenterology Cell Differentiation General Medicine Cell cycle Molecular biology Phenylbutyrates Growth Inhibitors Endocrinology Cancer cell |
| Zdroj: | World journal of gastroenterology. 18(1) |
| ISSN: | 2219-2840 |
| Popis: | AIM: To investigate the growth effects of 4-phenyl butyric acid (PBA) on human gastric carcinoma cells and their mechanisms. METHODS: Moderately-differentiated human gastric carcinoma SGC-7901 and lowly-differentiated MGC-803 cells were treated with 5, 10, 20, 40, and 60 μmol/L PBA for 1-4 d. Cell proliferation was detected using the MTT colorimetric assay. Cell cycle distributions were examined using flow cytometry. RESULTS: The proliferation of gastric carcinoma cells was inhibited by PBA in a dose- and time-dependent fashion. Flow cytometry showed that SGC-7901 cells treated with low concentrations of PBA were arrested at the G0/G1 phase, whereas cells treated with high concentrations of PBA were arrested at the G2/M phase. Although MGC-803 cells treated with low concentrations of PBA were also arrested at the G0/G1 phase, cells treated with high concentrations of PBA were arrested at the S phase. CONCLUSION: The growth inhibitory effect of PBA on gastric cancer cells is associated with alteration of the cell cycle. For moderately-differentiated gastric cancer cells, the cell cycle was arrested at the G0/G1 and G2/M phases. For lowly-differentiated gastric cancer cells, the cell cycle was arrested at the G0/G1 and S phases. |
| Databáze: | OpenAIRE |
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