Topically applied nitropyrenes are potent inducers of cutaneous and hepatic monooxygenases
| Autor: | Hasan Mukhtar, David R. Bickers, Herbert S. Rosenkranz, Mukul Das, Parthasarathi Asokan |
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| Rok vydání: | 1985 |
| Předmět: |
Time Factors
Cytochrome Biophysics 7-Alkoxycoumarin O-Dealkylase Pharmacology Biochemistry chemistry.chemical_compound Cytochrome P-450 Enzyme System Pregnancy medicine Cytochrome P-450 CYP1A1 Animals Inducer Enzyme inducer Epoxide hydrolase Molecular Biology Skin Epoxide Hydrolases Pyrenes biology Chemistry Cell Biology Monooxygenase Rats Spectrophotometry Enzyme Induction biology.protein Microsome Microsomes Liver Oxygenases Pyrene Female Aryl Hydrocarbon Hydroxylases Benzphetamine Oxidoreductases medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 129(1) |
| ISSN: | 0006-291X |
| Popis: | The inducibility of skin and liver microsomal cytochrome P-450 dependent aryl hydrocarbon hydroxylase and other monooxygenases by a mixture of nitropyrenes was assessed and compared with the parent non-nitrated compound, pyrene. A single topical application of nitropyrenes to neonatal rats resulted in highly significant induction of aryl hydrocarbon hydroxylase, ethoxycoumarin O-de-ethylase, and ethoxyresorufin O-de-ethylase activities in skin and liver after 24 hours. Inducibility of the skin and liver enzymes was 3.9–5.7 fold and 1.8–10.3 fold respectively. On the other hand, aminopyrine N-demethylase, benzphetamine N-demethylase and epoxide hydrolase activities in the liver were unaffected by topically applied nitropyrenes. Furthermore, treatment with nitropyrenes produced a 1 nm shift to the blue region in the wavelength maximum of hepatic microsomal cytochrome P-450. Topically applied pyrene produced only marginal or no effects on cutaneous and hepatic enzyme activities. Our results suggest that nitration of pyrene, a relatively ineffective enzyme inducer, produces nitropyrenes which are potent inducers of hepatic and cutaneous monooxygenases and they resemble 3-methylcholanthrene in this inducing effect. |
| Databáze: | OpenAIRE |
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