ERK Signaling Pathways Regulate the Osteogenic Differentiation of Human Mesenchymal Stem Cells on Collagen I and Vitronectin
| Autor: | Robert F. Klees, George E. Plopper, Mariah K Hughlock, Roman M. Salasznyk |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
MAPK/ERK pathway
MAP Kinase Signaling System Sialoglycoproteins Clinical Biochemistry Integrin Core Binding Factor Alpha 1 Subunit Antibodies Collagen Type I Extracellular matrix Cell Adhesion Humans Vitronectin Enzyme Inhibitors Phosphorylation Cells Cultured Flavonoids Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Osteoblasts biology Chemistry Integrin beta1 MEK inhibitor Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology General Medicine Alkaline Phosphatase Integrin alphaVbeta3 Cell biology Enzyme Activation RUNX2 Cancer research biology.protein Osteopontin Stem cell |
| Zdroj: | Cell Communication & Adhesion. 11:137-153 |
| ISSN: | 1543-5180 1541-9061 |
| Popis: | Adhesion to the extracellular matrix (ECM) proteins collagen I and vitronectin is sufficient to drive human mesenchymal stem cells (hMSCs) into an osteogenic differentiation pathway, but the mechanisms underlying this stimulation are not well understood. We found that addition of beta1 and alpha(v)beta3 integrin blocking antibodies inhibited ECM-induced ERK activation, while addition of the MEK inhibitor PD98059 blocked ERK activation, serine phosphorylation of the osteogenic transcription factor runx2/cbfa-1, osteogenic gene expression, and calcium deposition. These results suggest that ERK plays an important role in driving the ECM-induced osteogenic differentiation of hMSC. |
| Databáze: | OpenAIRE |
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