Isolation and phenotypic identification of non-tuberculous mycobacteria existing in Isfahan different water samples
| Autor: | Narimani Tahmineh, Moghim Shrareh, Safee Hagieh Ghasemian, Nasr Esfahani Bahram, Fazeli Hossein, Bahador Nima, Sadeghi Farzaneh, Faghri Jamshid, Sarikhani Ensieh, Zarkesh Fatemeh, Hoseini Nafiseh Sadat |
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| Rok vydání: | 2012 |
| Předmět: |
Veterinary medicine
biology business.industry Nitrate reduction test Incidence (epidemiology) lcsh:R Isfahan water samples lcsh:Medicine non tuberculous mycobacterium General Medicine Isolation (microbiology) biology.organism_classification phenotypic characteristics Phenotype Biotechnology Treatment management lcsh:Biology (General) Catalase biology.protein Medicine Original Article Catalase test business lcsh:QH301-705.5 Bacteria |
| Zdroj: | Advanced Biomedical Research Advanced Biomedical Research, Vol 1, Iss 1, Pp 18-18 (2012) |
| ISSN: | 2277-9175 |
| Popis: | Background: In recent decades, because of increasing prevalence of immunocompromised disease, non-tuberculous mycobacteria that have been known already as environmental bacteria presented as an important pathogens. In this study, the prevalence of NTM in Isfahan city water samples was determined bydifferent methods including culture methods with biochemical tests. Materials and Methods: Eighty-five water samples were collected from different sources in Isfahan, Iran. The mycobacterial isolates were identified by the growth characteristics, pigment production, semi-quantitative catalase test, Tween 80 hydrolysis, arylsulfatase test (3 and 14 days), heat-stable catalase (pH 7, 68°C), pyrazin amidase (4 and 7 days), urease, nitrate reduction test, and colony morphology. Results: Chlorine concentrations of the samples ranged from 0 to 1.8 mg/l. Twenty-one culturable species including M. fortuitum , 23.8% (5 cases); M.smegmatis , 14.3% (3 cases); M. chelonae like organisms, M. terrae complex, M. gordonae and M. mucogenicum , 9.5% (2 cases for each species); M. avium complex, M. phlei , M. xenopi, M. fallax , and M. flavescenc 4.8% (1 case for each species) were identified. Conclusion: The results of this study showed the incidence of different species of NTM in this geographical region in Iran. Studies show that the prevalence of immunocompromised disease is increasing in the community and it needs different treatment management strategy; therefore, the results of this study can be useful in this strategy. |
| Databáze: | OpenAIRE |
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