Irinotecan-Induced Mucositis Is Associated with Goblet Cell Dysregulation and Neural Cell Damage in a Tumour Bearing DA Rat Model
| Autor: | Daniel Thorpe, Masooma Sultani, Barbara Vanhoecke, Ross N. Butler, Andrea M. Stringer |
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| Přispěvatelé: | Thorpe, Daniel, Butler, Ross, Sultani, Masooma, Vanhoecke, Barbara, Stringer, Andrea |
| Rok vydání: | 2019 |
| Předmět: |
Mucositis
0301 basic medicine Cancer Research Pathology medicine.medical_specialty Antineoplastic Agents Irinotecan chemotherapy Enteric Nervous System Pathology and Forensic Medicine Jejunum 03 medical and health sciences enteric nervous system 0302 clinical medicine Intestinal mucosa mucus medicine Animals Intestinal Mucosa Myenteric plexus Neurons Goblet cell business.industry Neoplasms Experimental General Medicine medicine.disease Mucus Rats Disease Models Animal mucositis 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Female Enteric nervous system Goblet Cells business medicine.drug |
| Zdroj: | Pathology & Oncology Research. 26:955-965 |
| ISSN: | 1532-2807 1219-4956 |
| DOI: | 10.1007/s12253-019-00644-x |
| Popis: | Irinotecan–induced mucositis is a major oncological problem. Goblet cells secrete mucus, protecting the intestinal mucosa, with secretion altered during mucositis. The enteric nervous system is involved in regulating gut motility and secretion. The aim of this study was to determine whether enteric neural cells and goblet cells are altered following irinotecan treatment. Tumour-bearing Dark Agouti rats were administered a single dose of 175 mg/kg of irinotecan intraperitoneally and 0.01 mg/kg atropine subcutaneously. Experimental and untreated control rats were killed at times 6, 24, 48, 72, 96 and 120 h after treatment. Jejunum and colon samples were formalin fixed. Haematoxylin and eosin staining, Alcian Blue-PAS staining, and immunohistochemistry with S-100 antibody (neural cell marker) were carried out. Statistical analyses were carried out using Kruskal-Wallis test with Dunns post test, Mann Whitney U test and nonlinear regression. Total goblet cells decreased at 72 h compared with controls in the colon (p < 0.05). The percentage of cavitated goblet cells decreased compared to all other time points at 120 h in the colon. The number of S-100 positive cells in the submucosal plexus decreased in the colon (p = 0.0046) and in the myenteric plexus of the jejunum and colon (p = 0.0058 and p = 0.0022, respectively), when comparing treated with control. Enteric ganglia in the myenteric plexus of the jejunum decreased at 24 h and 96 h. Irinotecan-induced mucositis is associated with increases in mucus secretion, and enteric neural cell change. These changes may contribute to the pathophysiology of mucositis through the dysregulation of neural signalling Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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