Pinpointing pseurotins from a marine-derived Aspergillus as tools for chemical genetics using a synthetic lethality yeast screen
| Autor: | Karen Tenney, Jennifer E. Compton, Claudia M. Boot, Phillip Crews, Theodore R. Holman, Nadine C. Gassner, Craig M. Tamble, R. Scott Lokey |
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| Rok vydání: | 2007 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Stereochemistry Saccharomyces cerevisiae Pharmaceutical Science Sequence Homology Marine Biology Synthetic lethality Analytical Chemistry Aspergillus fumigatus Polyketide chemistry.chemical_compound Multienzyme Complexes Polyketide synthase Drug Discovery Candida albicans Peptide Synthases Nuclear Magnetic Resonance Biomolecular Pharmacology biology Molecular Structure Organic Chemistry Sequence Analysis DNA biology.organism_classification Yeast Pyrrolidinones Aspergillus Complementary and alternative medicine Biochemistry chemistry Pseurotin A biology.protein Molecular Medicine Chemical genetics Microtubule-Associated Proteins Polyketide Synthases |
| Zdroj: | Journal of natural products. 70(10) |
| ISSN: | 0163-3864 |
| Popis: | A new compound of mixed polyketide synthase-nonribosomal peptide synthetase (PKS/NRPS) origin, 11- O-methylpseurotin A ( 1), was identified from a marine-derived Aspergillus fumigatus. Bioassay-guided fractionation using a yeast halo assay with wild-type and cell cycle-related mutant strains of Saccharomyces cerevisiae resulted in the isolation of 1, which selectively inhibited a Hof1 deletion strain. Techniques including 1D and 2D NMR, HRESIMS, optical rotation, J-based analysis, and biosynthetic parallels were used in the elucidation of the planar structure and absolute configuration of 1. A related known compound, pseurotin A ( 2), was also isolated and found to be inactive in the yeast screen. |
| Databáze: | OpenAIRE |
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