Pre-steady-state Kinetic Analysis of Amino Acid Transporter SLC6A14 Reveals Rapid Turnover Rate and Substrate Translocation
| Autor: | Jiali Wang, Yueyue Shi, Christof Grewer, Elias Ndaru |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Alanine
chemistry.chemical_classification rapid kinetics Physiology Chemistry Kinetics Substrate (chemistry) ATB0 + Transporter electrophysiology Solute carrier family Amino acid molecular physiology laser-photolysis Physiology (medical) Biophysics QP1-981 Steady state (chemistry) Amino acid transporter SLC6A14 Original Research membrane transporter |
| Zdroj: | Frontiers in Physiology, Vol 12 (2021) Frontiers in Physiology |
| ISSN: | 1664-042X |
| DOI: | 10.3389/fphys.2021.777050 |
| Popis: | SLC6A14 (solute carrier family 6 member 14) is an amino acid transporter, driven by Na+ and Cl− co-transport, whose structure, function, and molecular and kinetic mechanism have not been well characterized. Its broad substrate selectivity, including neutral and cationic amino acids, differentiates it from other SLC6 family members, and its proposed involvement in nutrient transport in several cancers suggest that it could become an important drug target. In the present study, we investigated SLC6A14 function and its kinetic mechanism after expression in human embryonic kidney (HEK293) cells, including substrate specificity and voltage dependence under various ionic conditions. We applied rapid solution exchange, voltage jumps, and laser photolysis of caged alanine, allowing sub-millisecond temporal resolution, to study SLC6A14 steady state and pre-steady state kinetics. The results highlight the broad substrate specificity and suggest that extracellular chloride enhances substrate transport but is not required for transport. As in other SLC6 family members, Na+ binding to the substrate-free transporter (or conformational changes associated with it) is electrogenic and is likely rate limiting for transporter turnover. Transient current decaying with a time constant of |
| Databáze: | OpenAIRE |
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