A role for the transcription factor Helios in human CD4+CD25+ regulatory T cells
| Autor: | Tullia C. Bruno, Charles G. Drake, Joseph F. Grosso, Edward L. Hipkiss, Fan Pan, Monica V. Goldberg, Hung-Rong Yen, Derese Getnet, Timothy J. Harris, Satoshi Wada, Nicholas M. Durham, Drew M. Pardoll, Kristin J. Pyle |
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| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Regulatory T cell Immunology Down-Regulation Apoptosis HeliOS Biology DNA-binding protein Article Cell Line Ikaros Transcription Factor Mice Downregulation and upregulation medicine Animals Humans IL-2 receptor RNA Small Interfering Promoter Regions Genetic Molecular Biology Transcription factor Interleukin-2 Receptor alpha Subunit FOXP3 Forkhead Transcription Factors Molecular biology Up-Regulation DNA-Binding Proteins medicine.anatomical_structure Transcription Factors |
| Zdroj: | Molecular Immunology. 47:1595-1600 |
| ISSN: | 0161-5890 |
| Popis: | Relative upregulation of the Ikaros family transcription factor Helios in natural regulatory T cells (Tregs) has been reported by several groups. However, a role for Helios in regulatory T cells has not yet been described. Here, we show that Helios is upregulated in CD4(+)CD25(+) regulatory T cells. Chromatin-immunoprecipitation (ChIP) experiments indicated that Helios binds to the FoxP3 promoter. These data were further corroborated by experiments showing that knocking-down Helios with siRNA oligonucleotides results in down-regulation of FoxP3. Functionally, we found that suppression of Helios message in CD4(+)CD25(+) T cells significantly attenuates their suppressive function. Taken together, these data suggest that Helios may play an important role in regulatory T cell function and support the concept that Helios may be a novel target to manipulate Treg activity in a clinical setting. |
| Databáze: | OpenAIRE |
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