Production and in vitro characterization of 3D porous scaffolds made of magnesium carbonate apatite (MCA)/anionic collagen using a biomimetic approach
| Autor: | Racquel Z. LeGeros, Marcia S. Sader, Santiago Gomez, Gloria Dulce de Almeida Soares, Virginia da Conceição Amaro Martins |
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| Rok vydání: | 2013 |
| Předmět: |
Anions
Materials science Mineralogy chemistry.chemical_element Bioengineering Calcium Apatite Cell Line Biomaterials chemistry.chemical_compound X-Ray Diffraction COLÁGENO Biomimetics Apatites Spectroscopy Fourier Transform Infrared Bone cell Animals Humans chemistry.chemical_classification Tissue Scaffolds Magnesium Scleroprotein Buffer solution chemistry Chemical engineering Mechanics of Materials visual_art Microscopy Electron Scanning visual_art.visual_art_medium Cattle Collagen Crystallite Glutaraldehyde Powders Stromal Cells Porosity |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2013.06.006 |
| Popis: | 3D porous scaffolds are relevant biomaterials to bone engineering as they can be used as templates to tissue reconstruction. The aim of the present study was to produce and characterize in vitro 3D magnesium-carbonate apatite/collagen (MCA/col) scaffolds. They were prepared by using biomimetic approach, followed by cross-linking with 0.25% glutaraldehyde solution (GA) and liofilization. Results obtained with Fourier-transform infrared spectroscopy (FT-IR) confirmed the type-B carbonate substitution, while by X-ray diffraction (XRD), a crystallite size of ~10nm was obtained. Optical and electron microscopy showed that the cylindrical samples exhibited an open-porous morphology, with apatite nanocrystals precipitated on collagen fibrils. The cross-linked 3D scaffolds showed integrity when immersed in culture medium up to 14 days. Also, the immersion of such samples into an acid buffer solution, to mimic the osteoclastic resorption environment, promotes the release of important ions for bone repair, such as calcium, phosphorus and magnesium. Bone cells (SaOs2) adhered, and proliferated on the 3D composite scaffolds, showing that synthesis and the cross-linking processes did not induce cytotoxicity. |
| Databáze: | OpenAIRE |
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