Intensified Separation of Steviol Glycosides from a Crude Aqueous Extract of Stevia rebaudiana Leaves Using Centrifugal Partition Chromatography

Autor: Monique Berger, Joël Perret, Jean-Hugues Renault, Jean Daydé, Sébastien Chollet, Nicolas Borie, Claire Barbet-Massin, Jane Hubert
Přispěvatelé: Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Chimie des Substances Naturelles (ICMRCSN), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), CAPACITÉS, SAS, Physiologie, Pathologie et Génétique Végétales (PPGV), INP-PURPAN, Université Fédérale Toulouse Midi-Pyrénées, SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Stevia Natura, Partenaires INRAE, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2015
Předmět:
food.ingredient
[SDV]Life Sciences [q-bio]
Ethyl acetate
Pharmaceutical Science
Steviol
Centrifugation
natural sweeteners
Asteraceae
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
centrifugal partition chromatography
food
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Drug Discovery
Stevia
Stevioside
Glycosides
steviol glycosides
ComputingMilieux_MISCELLANEOUS
Pharmacology
chemistry.chemical_classification
Chromatography
010405 organic chemistry
Chemistry
[CHIM.ORGA]Chemical Sciences/Organic chemistry
Plant Extracts
Food additive
010401 analytical chemistry
Organic Chemistry
Glycoside
Water
Sweetness
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
6. Clean water
0104 chemical sciences
Stevia rebaudiana
Complementary and alternative medicine
Molecular Medicine
Diterpenes
Kaurane
Rebaudioside A
centrifugal partition extraction
Chromatography
Liquid
Zdroj: Planta Medica
Planta Medica, Georg Thieme Verlag, 2015, 81 (17), pp.1614-1620
Planta Medica, Georg Thieme Verlag, 2015, 81 (17), pp.1614-1620. ⟨10.1055/s-0035-1545840⟩
ISSN: 1439-0221
0032-0943
DOI: 10.1055/s-0035-1545840⟩
Popis: International audience; Aqueous extracts of Stevia rebaudiana leaves have been approved since 2008 by the Joint Expert Committee for Food Additives as sugar substitutes in many food and beverages in Western and Far East Asian countries. The compounds responsible for the natural sweetness of Stevia leaves include a diversity of diterpenoid glycosides derived from a steviol skeleton. These steviol glycosides also exhibit a low calorific value as well as promising therapeutic applications, particularly for the treatment of sugar metabolism disturbances. In this work, centrifugal partition chromatography is proposed as an efficient technical alternative to purify steviol glycosides from crude aqueous extracts of Stevia leaves on a multigram scale. Two different commercial instruments, including an ASCPC250 (R) and a FCPE300 (R) made of columns containing 1890 and 231 twin-cells, respectively, were evaluated and compared. All experiments were performed with a polar biphasic solvent system composed of ethyl acetate, n-butanol, and water in a gradient elution mode. When using the 1890 partition cell centrifugal partition chromatography column of 250 mL, 42 mg of stevioside, 68 mg of dulcoside A, and 172 mg of rebaudioside A, three major constituents of the initial extract were obtained from 1 g of the initial mixture at purities of 81%, 83%, and 99%, respectively. The productivity was further improved by intensifying the procedure on the 231 partition cell centrifugal partition chromatography column of 303 mL with the sample mass loading increased up to 5 g, resulting in the recovery of 1.2 g of stevioside, 100 mg of dulcoside A, and 1.1 g of rebaudioside A at purities of 79%, 62%, and 98%, respectively. The structures of the isolated compounds were validated by HPLC-UV, ESI-MS, H-1, and C-13 NMR analyses. Altogether, the results demonstrate that the column design (i.e., the partition cell number) is an important aspect to be considered for a larger scale centrifugal partition chromatography isolation of Stevia-derived natural sweeteners.
Databáze: OpenAIRE
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