The determination of the potential anticancer effects of Coriandrum sativum in PC-3 and LNCaP prostate cancer cell lines
| Autor: | Mücahit Seçme, Levent Elmas, Umut Fahrioglu, Yavuz Dodurga, Ramazan Mammadov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine cell migration Coriandrum protein p53 Apoptosis IC50 urologic and male genital diseases Biochemistry apoptotic protease activating factor 1 Prostate cancer coriander 0302 clinical medicine Sativum Cell Movement Tumor Cells Cultured caspase 9 prostate cancer cell line biology Chemistry LNCaP Cell migration prostate cancer cell invasion PC-3 [Human prostate carcinoma] cell line Gene Expression Regulation Neoplastic PUMA protein priority journal 030220 oncology & carcinogenesis herbal medicine cytotoxicity protein bcl 2 in vitro study Antineoplastic Agents caspase 10 antineoplastic activity colony formation protein Noxa Article 03 medical and health sciences PC-3 Biomarkers Tumor medicine Humans PTEN death receptor 5 controlled study Neoplasm Invasiveness death receptor 4 protein Bid human tumor necrosis factor receptor associated death domain protein Molecular Biology Protein kinase B cell viability Cell Proliferation Wound Healing Plant Extracts human cell Coriandrum sativum extract Prostatic Neoplasms phosphatidylinositol 3 4 5 trisphosphate 3 phosphatase Cell Biology medicine.disease biology.organism_classification LNCaP cell line 030104 developmental biology Cell culture gene expression Cancer research biology.protein protein kinase B |
| Popis: | Coriander (Coriandrum sativum L.) is such an herb from the Apiaceae family, used both for its medicinal and nutritional properties for many centuries. In this study, the effects of C. sativum extract on gene expression, viability, colony formation, migration, and invasion of PC-3 and LNCaP prostate cancer cell lines have been investigated. The half maximal inhibitory concentration (IC 50 ) dose in PC-3 and LNCaP cells was detected to be 2 and 5 mg/mL at the 24th hour, respectively. C. sativum extracts have been observed to cause a significant decrease in the expression of Akt and Bcl-2 in the PC-3 cells and just Akt in LNCaP cells while increasing in the expression of p53, caspase-9, caspase-10, PTEN, DR5, TRADD, PUMA, and NOXA. DR4 expression was increased in LNCaP cell line but not PC-3, and APAF and BID had increased expression in PC-3 but not the LNCaP cells. Our observations have shown that C. sativum extract decreased colony formation while inhibiting cell invasion and migration. Cell migration was hindered in PC-3 but not the LNCaP cells. In conclusion, this data present a valuable addition to the very limited data available out there on the potential use of C. sativum in prostate cancer treatment. © 2018 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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