Opening of ATP-sensitive K + (K ATP ) channels enhance hydroxyl radical generation induced by MPP + in rat striatum
| Autor: | Michiko Nakashima, Toshio Obata |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Cromakalim Microdialysis Dopamine Glibenclamide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine KATP Channels Membrane Transport Modulators Glyburide Hydroxybenzoates medicine Animals Rats Wistar Nicorandil Sodium salicylate Dose-Response Relationship Drug Hydroxyl Radical MPTP MPTP Poisoning Corpus Striatum 030104 developmental biology Neurology chemistry Biochemistry Biophysics Hydroxyl radical Neurology (clinical) Adenosine triphosphate 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of the Neurological Sciences. 366:180-183 |
| ISSN: | 0022-510X |
| DOI: | 10.1016/j.jns.2016.04.004 |
| Popis: | The present study examined whether opening of adenosine triphosphate (ATP) sensitive K(+) (KATP) channels can enhance 1-methyl-4-phenylpyridinium (MPP(+))-induced hydroxyl radical (OH) generation in rat striatum. Rats were anesthetized, and sodium salicylate in Ringer's solution (0.5nmol/ml per min) was infused through a microdialysis probe to detect the generation of OH as reflected by the non-enzymatic formation of 2.3-dihydroxybenzoic acid (DHBA) in the striatum. MPP(+) (5mM) enhanced generation of OH with concomitant increased efflux of dopamine (DA). Cromakalim (100μM), a KATP channel opener, through the microdialysis probe significantly increased both DA efflux and OH formation induced by MPP(+). Another KATP channel opener, nicorandil (1mM), also increased the level DA or DHBA, but these changes were not significant. However, in the presence of glibenclamide (10μM), a KATP channel antagonist, and the increase of MPP(+)-induced DA or DHBA were not observed. Cromakalim (10, 50 and 100μM) increased MPP(+)-induced DHBA formation in a concentration-dependent manner. However, the effects of cromakalim in the presence of glibenclamide were abolished. These results suggest that opening of KATP channels may cause OH generation by MPP(+). |
| Databáze: | OpenAIRE |
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