Retinal homeobox 1 is required for retinal neurogenesis and photoreceptor differentiation in embryonic zebrafish
| Autor: | Leon Park, Steve M. Nelson, Deborah L. Stenkamp |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Embryo
Nonmammalian genetic structures Neurogenesis Morpholino Eye Retina Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Photoreceptor Cells Rod Zebrafish Molecular Biology In Situ Hybridization 030304 developmental biology Homeodomain Proteins 0303 health sciences Gene knockdown Photoreceptor biology Retinal Cell Differentiation Cell Biology Zebrafish Proteins biology.organism_classification Molecular biology Immunohistochemistry eye diseases Cell biology medicine.anatomical_structure chemistry Eye development Homeobox rx/rax PAX6 sense organs Transcription factor 030217 neurology & neurosurgery Cone Progenitor Developmental Biology |
| Zdroj: | Developmental Biology. 328(1):24-39 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2008.12.040 |
| Popis: | Retinal homeobox (Rx/Rax) genes are essential for the organogenesis of the vertebrate eye. These genes are dynamically expressed in a tissue-specific manner during eye development, suggesting pleiotropic roles. We use a temporally-selective gene knockdown approach to identify endogenous functions for the zebrafish rx genes, rx1 and rx2. Depletion of rx1 over the period of eye organogenesis resulted in severely reduced proliferation of retinal progenitors, the loss of expression of the transcription factor pax6, delayed retinal neurogenesis, and extensive retinal cell death. In contrast, depletion of rx2 over the same developmental time resulted in reduced expression of pax6 in the eye anlage, but only modest effects on retinal cell survival. Knockdown of rx1 specifically during photoreceptor development inhibited the expression of multiple photoreceptor-specific genes, while knockdown of rx2 over this time selectively inhibited the expression of a subset of these genes. Our findings support a function for rx2 in regulating pax6 within the optic primordia, a function for rx1 in maintaining the pluripotent, retinal progenitor cell state during retinal development, as well as selective functions for rx1 and rx2 in regulating photoreceptor differentiation. |
| Databáze: | OpenAIRE |
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