Binary IS Typing for Staphylococcus aureus
| Autor: | Budding, A.E., Vandenbroucke-Grauls, C.M.J.E., Melles, D.C., van Duijkeren, E., Kluytmans, J., Savelkoul, P.H., Advances in Veterinary Medicine, Dep Infectieziekten Immunologie |
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| Přispěvatelé: | Medical Microbiology and Infection Prevention, CCA - Immuno-pathogenesis, Advances in Veterinary Medicine, Dep Infectieziekten Immunologie, Medical Microbiology & Infectious Diseases |
| Rok vydání: | 2010 |
| Předmět: |
Infectious Diseases/Epidemiology and Control of Infectious Diseases
Staphylococcus aureus Public Health and Epidemiology/Infectious Diseases lcsh:Medicine Locus (genetics) Biology medicine.disease_cause DNA Ribosomal Public Health and Epidemiology/Nosocomial and Healthcare-Associated Infections law.invention law medicine Typing Amplified Fragment Length Polymorphism Analysis lcsh:Science Polymerase chain reaction Genetics Multidisciplinary lcsh:R Microbiology/Medical Microbiology food and beverages Infectious Diseases/Nosocomial and Healthcare-Associated Infections bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus Multilocus sequence typing Amplified fragment length polymorphism lcsh:Q Research Article |
| Zdroj: | PLoS ONE, 5(10). Public Library of Science PLoS ONE, Vol 5, Iss 10, p e13671 (2010) PLoS One (print), 5(10). Public Library of Science Budding, A E, Vandenbroucke-Grauls, C M J E, Melles, D C, van Duijkeren, E, Kluijtmans, J A J W & Savelkoul, P H M 2010, ' Binary IS Typing for Staphylococcus aureus ', PLoS ONE, vol. 5, no. 10, pp. e13671 . https://doi.org/10.1371/journal.pone.0013671 PLoS One, 5(10). Public Library of Science PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background: We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus. Methodology/Principal Findings: A novel approach of IS-typing was performed in which binary profiles are created. 424 human and animal derived MRSA and MSSA isolates were tested and a subset of these isolates was compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP). Binary IS typing had a high discriminatory potential and a good correlation with MLST and AFLP. Conclusions/Significance: Binary IS typing is easy to perform and binary profiles can be generated in a standardized fashion. These two features, combined with the high correlation with MLST clonal complexes, make the technique applicable for large-scale inter-laboratory molecular epidemiological comparisons. |
| Databáze: | OpenAIRE |
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