Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A2 isozyme genes
| Autor: | Takahito Chijiwa, Yoshiyuki Sakaki, Hitomi Nakamura, Shosaku Hattori, Tatsuo Murakami, Takatoshi Ohkuri, Naoko Oda-Ueda, Motonori Ohno |
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| Rok vydání: | 2014 |
| Předmět: |
DNA
Complementary TATA box Genetic Vectors Molecular Sequence Data Electrophoretic Mobility Shift Assay Venom CHO Cells Toxicology complex mixtures Cricetulus Exocrine Glands Japan Viperidae Cricetinae biology.animal Crotalid Venoms Animals Luciferases Promoter Regions Genetic Transcription factor DNA Primers Expressed Sequence Tags Base Sequence Proto-Oncogene Proteins c-ets biology Reverse Transcriptase Polymerase Chain Reaction ETS transcription factor family Promoter Sequence Analysis DNA Venom Protein Molecular biology Enzyme Activation Isoenzymes Phospholipases A2 Snake venom Fluorescein-5-isothiocyanate |
| Zdroj: | Toxicon. 92:133-139 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/j.toxicon.2014.10.001 |
| Popis: | Protobothrops flavoviridis (habu) (Crotalinae, Viperidae) is a Japanese venomous snake, and its venom contains the enzymes with a variety of physiological activities. The phospholipases A2 (PLA2s) are the major components and exert various toxic effects. They are expressed abundantly in the venom gland. It is thought that the venom gland-specific transcription factors play a key role for activation of PLA2 genes specifically expressed in the venom gland. Thus, the full-length cDNA library for P. flavoviridis venom gland after milking of the venom was made to explore the transcription factors therein. As a result, three cDNAs encoding epithelium-specific ETS transcription factors (ESE)-1, -2, and -3 were obtained. Among them, ESE-3 was specifically expressed in the venom gland and activated the proximal promoters of venom PLA2 genes, which are possibly regarded as the representatives of the venom gland-specific protein genes in P. flavoviridis. Interestingly, the binding specificity of ESE-3 to the ETS binding motif located near TATA box is well correlated with transcriptional activities for the venom PLA2 genes. This is the first report that venom gland-specific transcription factor could actually activate the promoters of the venom protein genes. |
| Databáze: | OpenAIRE |
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