Phyllanthin of Standardized Phyllanthus amarus Extract Attenuates Liver Oxidative Stress in Mice and Exerts Cytoprotective Activity on Human Hepatoma Cell Line
| Autor: | Lonchin Suguna, Rajesh Krithika, Ramtej J Verma, Pranav S. Shrivastav |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Liver injury
Hepatology business.industry Hepatotoxin Pharmacology Malondialdehyde medicine.disease medicine.disease_cause Lipid peroxidation chemistry.chemical_compound chemistry Phytochemical Hepatoprotection Biochemistry Medicine Original Article High performance thin layer chromatography business Oxidative stress |
| Popis: | Background Phyllanthus amarus, a traditional herbal liver-protecting medicine, is known to contain an active ingredient phyllanthin. Many research studies and clinical trials performed in the past using this plant have given contentious results which clearly accentuates the need for the standardization of the extracts. Aim In this study, P. amarus extract was standardized for phyllanthin content by high performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) analysis. The preventive role of a standardized extract of P. amarus against CC1 4 -induced hepatotoxicity in vivo and in vitro using mice model and human hepatoma HepG2 cell line, respectively, was investigated. Methods Phyllanthin was used as a marker phytochemical for the standardization of P. amarus extract. The extracts were verified for phyllanthin content by HPTLC and HPLC. Female mice were orally administered with CCl 4 either with or without standardized P. amarus extract in three different doses. Similarly, the cytoprotective role of the standardized extract in vitro was studied in HepG2 cell line. Results Oral administration of CCl 4 resulted in increased oxidative stress, decreased antioxidative defense, and liver injury. Treatment with P. amarus along with CCl 4 significantly mitigated the increase in activities of liver marker enzymes, lipid peroxidation, and bilirubin content. It also increased the antioxidant enzymatic and non-enzymatic defense parameter levels. The results of the in vitro study conducted in HepG2 cells indicated that the hepatotoxin lowered 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Mil) metabolism and increased the release of transaminases which were corrected with co-incubation with P. amarus. Conclusion: The study established a significant liver-protecting role of standardized P. amarus extract due to the presence of active ingredient phyllanthin. |
| Databáze: | OpenAIRE |
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