WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis
| Autor: | Han Rauwerda, Silke Allmann, Maaike R. Boersma, Michel de Vries, Robert C. Schuurink, Alessandra Scala, Eleni A. Spyropoulou, Michel A. Haring, Rossana Mirabella, Timo M. Breit |
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| Přispěvatelé: | Plant Physiology (SILS, FNWI), RNA Biology & Applied Bioinformatics (SILS, FNWI) |
| Rok vydání: | 2015 |
| Předmět: |
Glutamate decarboxylase
Mutant Arabidopsis Plant Science Regulatory Sequences Nucleic Acid Plant Roots Gene Expression Regulation Plant Botany Genetics Transcription factor gamma-Aminobutyric Acid Aldehydes biology Arabidopsis Proteins Gene Expression Profiling Green leaf volatiles Promoter Cell Biology biology.organism_classification WRKY protein domain Cell biology Plant Leaves Mutation Trans-acting Transcription Factors |
| Zdroj: | Plant Journal, 83(6), 1082-1096. Wiley-Blackwell |
| ISSN: | 1365-313X 0960-7412 |
| Popis: | Plants are known to be responsive to volatiles, but knowledge about the molecular players involved in transducing their perception remains scarce. We study the response of Arabidopsis thaliana to E-2-hexenal, one of the green leaf volatiles (GLV) that is produced upon wounding, herbivory or infection with pathogens. We have taken a transcriptomics approach to identify genes that are induced by E-2-hexenal, but not by defence hormones or other GLVs. Furthermore, by studying the promoters of early E-2-hexenal-induced genes we determined that the only statistically enriched cis-element was the W-box motif. Since members of the plant-specific family of WRKY transcription factors act in trans on this cis-element, we focused on WRKY6, 40 and 53 that were most strongly induced by E-2-hexenal. Root elongation of Arabidopsis seedlings of the wrky40 wrky6 double mutant was much less inhibited than in wt plants, similar to the E-2-hexenal-responsive mutant her1, which is perturbed in gamma-amino butyric acid (GABA) metabolism. The induction of several of the E-2-hexenal-specific genes was much higher in the wrky40, wrky6 or wrky40 wrky6 mutants, including GAD4, a glutamate decarboxylase that catalyzes the formation of GABA from glutamate. In conclusion, WRKY6 and 40 seem to act as important players transducing E-2-hexenal perception. |
| Databáze: | OpenAIRE |
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