Incorporation of dimethyl sulfoxide into experimental hydrophilic and hydrophobic adhesive resins: evaluation of cytotoxic activities
| Autor: | R. Seseogullari-Dirihan, Anas Aaqel Salim Salim Al-Ani, Ikram Aqel Salim, Murat Mutluay, Arzu Tezvergil-Mutluay, Leo Tjäderhane |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Biocompatibility
0206 medical engineering Dental Cements 02 engineering and technology Methacrylate Composite Resins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system Materials Testing Dentin medicine Humans Dimethyl Sulfoxide Viability assay Cytotoxicity General Dentistry Dimethyl sulfoxide technology industry and agriculture Dental Bonding 030206 dentistry 020601 biomedical engineering Resin Cements medicine.anatomical_structure chemistry Dentin-Bonding Agents Pulp (tooth) Methacrylates Adhesive Hydrophobic and Hydrophilic Interactions Nuclear chemistry |
| Zdroj: | European journal of oral sciencesREFERENCES. 129(1) |
| ISSN: | 1600-0722 |
| Popis: | This study evaluated the cytotoxicity of methacrylate-based resins containing dimethyl sulfoxide (DMSO). DMSO was incorporated into hydrophobic (R2) and hydrophilic (R5) resins at weight concentrations of 0, 0.01, 0.1, 1, 5, or 10 w/w %. Resin discs (n = 10/group) were prepared. Human gingival fibroblasts (HGF-1) were exposed to resin eluates for 24 h. Furthermore, dentin barrier test was performed using 3-D cultures of odontoblast-like cells (SV40 transfected pulp derived cells) with dentin slices of 400 µm thickness (n = 8). After acid etching of dentin, DMSO-modified resins were applied into the cavity part of the device and light-cured for 20 s. Cell viability (%) was assessed by MTT and analyzed spectrometrically. Data were analyzed by ANOVA and Tukey test (α = 0.05). Resin eluates showed statistically significantly lower % cell viability for all neat and DMSO-modified resins than seen for the negative control. Moreover, DMSO-R5 eluates resulted in significantly lower % cell viability than DMSO-R2 emulates. The dentin barrier test showed that DMSO-R2 did not result in significantly lower % cell viability, whereas incorporation of 1-10 w/w % DMSO into R5 resulted in significantly lower % of cell viability. Incorporating DMSO into hydrophilic self-etching resins may increase cytotoxicity. The biocompatibility is not influenced by the addition of DMSO into hydrophobic resin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text