An Unexpected Regulatory Cascade Governs a Core Function of the Drosophila PRC1 Chromatin Protein Su(z)2
| Autor: | Stephanie Yu, Son C. Nguyen, Elaine M. Oberlick, Chao-ting Wu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics 030102 biochemistry & molecular biology fungi Protein domain Polycomb-Group Proteins Investigations Biology Posterior Sex Combs DNA-binding protein Chromatin Protein–protein interaction DNA-Binding Proteins 03 medical and health sciences 030104 developmental biology Protein Domains Polycomb-group proteins Animals Drosophila Proteins Drosophila PRC1 Drosophila Protein |
| Zdroj: | Genetics. 205:551-558 |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.116.187849 |
| Popis: | Polycomb group (PcG) proteins are major chromatin-bound factors that can read and modify chromatin states to maintain gene silencing throughout development. Here we focus on a close homolog of the PcG protein Posterior sex combs to better understand how these proteins affect regulation. This homolog, called Suppressor 2 of zeste [Su(z)2] is composed of two regions: the N-terminal homology region (HR), which serves as a hub for protein interactions, and the C-terminal region (CTR), which is believed to harbor the core activity of compacting chromatin. Here, we describe our classical genetic studies to dissect the structure of Su(z)2. Surprisingly, we found that the CTR is dispensable for viability. Furthermore, the core activity of Su(z)2 seems to reside in the HR instead of the CTR. Remarkably, our data also suggest a regulatory cascade between CTR and HR of Su(z)2, which, in turn, may help prioritize the myriad of PcG interactions that occur with the HR. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|