Antibacterial and cytotoxic effect of honey mediated copper nanoparticles synthesized using ultrasonic assistance

Autor: Jactty Chew, Nur Afini Ismail, Siti Nur Amalina Mohamad Sukri, Kamyar Shameli, Magdelyn Mei-Theng Wong, Sin-Yeang Teow
Rok vydání: 2019
Předmět:
Copper oxide
Materials science
Metal Nanoparticles
Nanoparticle
chemistry.chemical_element
Bioengineering
Fructose
Microbial Sensitivity Tests
02 engineering and technology
010402 general chemistry
01 natural sciences
Enterococcus faecalis
Biomaterials
chemistry.chemical_compound
X-Ray Diffraction
Cell Line
Tumor
Spectroscopy
Fourier Transform Infrared
Escherichia coli
Humans
Ultrasonics
Fourier transform infrared spectroscopy
High-resolution transmission electron microscopy
Microbial Viability
Cell Death
biology
technology
industry
and agriculture
Spectrometry
X-Ray Emission
Honey
021001 nanoscience & nanotechnology
biology.organism_classification
Ascorbic acid
Copper
Anti-Bacterial Agents
0104 chemical sciences
Glucose
chemistry
Mechanics of Materials
Spectrophotometry
Ultraviolet
0210 nano-technology
Antibacterial activity
Nuclear chemistry
Zdroj: Materials Science and Engineering: C. 104:109899
ISSN: 0928-4931
DOI: 10.1016/j.msec.2019.109899
Popis: In this study, a comparative study of effect using honey on copper nanoparticles (Cu-NPs) via simple, environmentally friendly process and inexpensive route was reported. Honey and ascorbic acid act as stabilizing and reducing agents with the assistance of sonochemical method. The products were characterized using UV–visible (UV–vis) spectroscopy, X-Ray Diffraction (XRD), High-Resolution Transmission Electron Microscopy (HRTEM), Field-Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared (FTIR) spectroscopy. The reddish brown colour demonstrated the formation of Cu-NPs and UV–visible proved the plasmon resonance of Cu-NPs. XRD also confirmed a highly pure Cu-NPs obtained with absence of copper oxide in which the structure is crystalline. The spherical size of the Cu-NPs was acquire in the presence of honey which is 3.68 ± 0.78 nm with narrow particle distribution. The antibacterial activity was seen against gram-positive and gram-negative bacteria which are Enterococcus faecalis (E. faecalis) and Escherichia coli (E. coli). At higher concentration of Cu-NPs, they were more effective in killing both bacteria. The Cu-NPs without and with honey exhibited toxicities toward normal and cancerous cells. However, Cu-NPs without honey showed more potent killing activity against normal and cancer cells.
Databáze: OpenAIRE
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