Evaluation of the effects of two anaesthetic protocols on oxidative status and DNA damage in red-eared sliders (Trachemys scripta elegans) undergoing endoscopic coeliotomy
| Autor: | Milena Radaković, Maja Vasiljević, Zoran Stanimirovic, Miloš Vučićević, Darko Marinković, Milan Došenović, Branislav Vejnović |
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| Rok vydání: | 2021 |
| Předmět: |
040301 veterinary sciences
DNA damage Oxidative phosphorylation Pharmacology 030308 mycology & parasitology 0403 veterinary science Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound medicine Animals Anesthetics 0303 health sciences General Veterinary biology business.industry Endoscopy 04 agricultural and veterinary sciences Glutathione Malondialdehyde Turtles Comet assay Oxidative Stress chemistry Catalase biology.protein Propofol business DNA Damage medicine.drug |
| Zdroj: | Acta Veterinaria Hungarica |
| ISSN: | 1588-2705 0236-6290 |
| DOI: | 10.1556/004.2020.00058 |
| Popis: | The aim of this study was to assess how red-eared sliders (Trachemys scripta elegans) respond to anaesthesia itself and coelioscopy. For that purpose, the turtles were anaesthetised with ketamine–medetomidine or propofol, and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) and the level of malondialdehyde (MDA) were determined by spectrophotometry. The possible genotoxic effects of the anaesthetic agents were estimated by comet assay. A total of 24 turtles were included in this study. The animals were divided into four groups according to the anaesthetic protocol and according to whether endoscopy would be performed. Significantly decreased activities of CAT were found only in the propofol group and in turtles undergoing coelioscopy. Both anaesthetic protocols induced significantly increased MDA levels, while no differences were observed after the intervention. A significant increase in GST activity was detected in turtles after both anaesthetic protocols, but after coelioscopy significant changes in GST activity were found only in the propofol group. However, no differences in SOD activity and no DNA damages were detected in either group. These findings suggest that ketamine–medetomidine may be more suitable anaesthetic agents in red-eared sliders than propofol. |
| Databáze: | OpenAIRE |
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