Sequence Characterization of a Novel α-Neurotoxin from the King Cobra (Ophiophagus hannah) Venom
| Autor: | Kuo-Wha Kuo, Kai-Fa Huang, Shun-Wen Chen, Shyh-Horng Chiou, Tai-Ying Huang, Chun-Chang Chang |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
King cobra
Protein Conformation King Cobra Venom Molecular Sequence Data Neurotoxins Biophysics Venom Receptors Nicotinic Torpedo Biochemistry Protein Structure Secondary Lysyl endopeptidase Endopeptidases medicine Animals Neurotoxin Amino Acid Sequence Molecular Biology Peptide sequence Chromatography High Pressure Liquid Elapid Venoms Electric Organ Sequence Homology Amino Acid biology Edman degradation Snakes Cell Biology Chromatography Ion Exchange biology.organism_classification Trypsin Peptide Fragments Chromatography Gel Electrophoresis Polyacrylamide Gel medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 191:214-223 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.1205 |
| Popis: | Several postsynaptic neurotoxins (α-neurotoxins) with distinct pharmacological and biochemical properties were isolated and purified from the King cobra venom ( Ophiophagus hannah ) by employing sequentially preparative-scale cation-exchange chromatography on SP-Sephadex C-25 coupled with gel filtration and reversed-phase HPLC. The complete sequence of one neurotoxin was determined by N-terminal Edman degradation with the automatic pulsed-liquid phase sequencer on some peptide fragments generated from the endopeptidases, i . e . trypsin, S . aureus V 8 protease and lysyl endopeptidase. This novel neurotoxin is a basic polypeptide of pI 9.05, consisting of 72 amino-acid residues with 10 cysteine residues. It is found to share about 60 % sequence homology with Toxins a and b isolated from the same venom and the well established α-bungarotoxin, a major postsynaptic toxic ligand for acetylcholine receptor isolated from Bungarus multicinctus . The characterized α-neurotoxin molecules were also shown to bind specifically with nicotinic acetylcholine receptors of the electric eel, Torpedo californica . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|