High-throughput miRNA sequencing of the human placenta: expression throughout gestation
| Autor: | Erica Sauro, Yayu Lin, Jie Tang, Rae A. Buttle, Yizhou Wang, Tania L Gonzalez, N. Joshi, Yazhen Zhu, Jessica L. Chan, Rosemarie DiPentino, John Williams, Kent D. Taylor, Amy E Flowers, Margareta D. Pisarska, Di Wu, Laura E Eisman, Ekaterina L Clark, Yalda Afshar, Caroline A. Jefferies, Hsian-Rong Tseng, Chintda Santiskulvong |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Placenta Gestational Age Biology Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy Chromosome 19 microRNA Genetics medicine Humans Epigenetics Chromosome 13 Gene Expression Profiling Pregnancy Outcome Computational Biology High-Throughput Nucleotide Sequencing medicine.disease Fold change MicroRNAs 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis Gestation Female Transcriptome Biomarkers Research Article |
| Zdroj: | Epigenomics |
| ISSN: | 1750-192X |
| Popis: | Aim: To understand miRNA changes across gestation in healthy human placentae. This is essential before miRNAs can be used as biomarkers or prognostic indicators during pregnancy. Materials & methods: Using next-generation sequencing, we characterize the normative human placenta miRNome in first (n = 113) and third trimester (n = 47). Results & conclusion: There are 801 miRNAs expressed in both first and third trimester, including 182 with similar expression across gestation (p ≥ 0.05, fold change ≤2) and 180 significantly different (false discovery rate 2). Of placenta-specific miRNA clusters, chromosome 14 miRNA cluster decreases across gestation and chromosome 19 miRNA cluster is overall highly expressed. Chromosome 13 clusters are upregulated in first trimester. This work provides a rich atlas of healthy pregnancies to direct functional studies investigating the epigenetic differences in first and third trimester placentae. |
| Databáze: | OpenAIRE |
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