Localization of a hepatitis B surface antigen determinant deduced from results of chemical modifications
| Autor: | A.R. Neurath, Nathan Strick, W. R. Oleszko |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Antigenicity
HBsAg Methionine Hepatitis B Surface Antigens Tunicamycin Lysine Tryptophan Carbohydrates virus diseases Biology Molecular biology digestive system diseases Epitope chemistry.chemical_compound Carbodiimides Epitopes Biochemistry chemistry Virology Amino Acid Sequence Cyanogen Bromide Peptide sequence Cysteine |
| Zdroj: | Journal of virological methods. 3(2) |
| ISSN: | 0166-0934 |
| Popis: | The effect on hepatitis B surface antigen (HBsAg) of reagents considered specific for each of the amino acid residues, lysine, methionine, cysteine, arginine, tyrosine, tryptophan, and glutamic (aspartic) acid, was studied. Based on the observed alterations of HBsAg antigenicity and the known amino acid sequence of the HBsAg polypeptide, a major antigenic determinant was localized within the sequence: Pro-Ser-Cys-Cys-Cys-Thr-Lys-Pro-Thr(Ser)-Asp-Gly-Asn-Cys-Thr-Cys-Ile-Pro-Ile-Pr o-Ser-Ser, corresponding to residues 135-155. The asparagine-linked saccharide chains are not essential for HBsAg antigenicity. |
| Databáze: | OpenAIRE |
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