In-cell click labelling of small molecules to determine subcellular localisation

Autor:	Owen Everson, Tram T. Tran, Emily J. S. Graham, Florian Wakenhut, Paul Targett-Adams, Jared Millbank, David M. Beal, Lyn H. Jones, Matthew D. Selby, George M. Burslem, Peter G. Dodd
Rok vydání:	2010
Předmět:	Fluorophore Chemistry viruses Biophysics Cell Biology biochemical phenomena metabolism and nutrition Biochemistry Small molecule Article chemistry.chemical_compound Click chemistry Molecule Replicon Azide Mode of action NS5A
Zdroj:	Journal of Chemical Biology. 4:49-53
ISSN:	1864-6166 1864-6158
DOI:	10.1007/s12154-010-0047-1
Popis:	Small molecule fluorometric boron dipyrromethene probes were developed to bind hepatitis C virus-encoded NS5A protein and aid subcellular distribution studies. These molecules did not co-locate with NS5A, therefore alternative 'silent' azide reporters were used to obtain a more relevant picture of their distribution. Following pre-incubation with replicon cells, click chemistry was used to append a fluorophore to the azide that confirmed the co-localisation of the small molecule with the NS5A protein, thus providing greater insight into the antiviral mode of action of this chemotype.The online version of this article (doi:10.1007/s12154-010-0047-1) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c61f9cdb1e900ddd76f1a0a2b58161d https://doi.org/10.1007/s12154-010-0047-1 Zobrazit plný text záznamu Full text from SpringerLink