Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion†
| Autor: | Masashi Takahashi, Ahmed Z. Balboula, Karen Schindler, Amanda S. Gentilello, Cecilia S. Blengini |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cycloheximide Biology Mice 03 medical and health sciences chemistry.chemical_compound Meiosis Gamete Biology medicine Animals Aurora Kinase C Cloning Molecular RNA Translation (biology) Cell Biology General Medicine Oocyte Cell biology RNA Messenger Stored 030104 developmental biology medicine.anatomical_structure Reproductive Medicine chemistry Protein Biosynthesis Oocytes Female Multipolar spindles Cytokinesis |
| Zdroj: | Biology of Reproduction. 96:1197-1209 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1093/biolre/iox047 |
| Popis: | During oocyte meiotic maturation, Aurora kinase C (AURKC) is required to accomplish many critical functions including destabilizing erroneous kinetochore–microtubule (K-MT)attachments and regulating bipolar spindle assembly. How localized activity of AURKC is regulated in mammalian oocytes, however, is not fully understood. Female gametes from many species, including mouse, contain stores of maternal transcripts that are required for downstream developmental events. We show here that depletion of maternal RNA in mouse oocytes resulted in impaired meiotic progression, increased incidence of chromosome misalignment and abnormal spindle formation at metaphase I (Met I), and cytokinesis defects. Importantly, depletion of maternal RNA perturbed the localization and activity of AURKC within the chromosomal passenger complex (CPC). These perturbations were not observed when translation was inhibited by cycloheximide (CHX) treatment. These results demonstrate a translation-independent function of maternal RNA to regulate AURKC-CPC function in mouse oocytes. |
| Databáze: | OpenAIRE |
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